http://www.desmech.com An Express Engineering Blog Mon, 20 Apr 2009 08:36:05 +0000 http://backend.userland.com/rss092 en Tangential Flow Filtration (TFF) is used extensively in biopharmaceutical industries for clarification of microbial cells/animal cells, inclusion bodies clarification, protein concentration and diafiltration, buffer exchange, viral clearance, etc. Multiple Centrifugation steps can be avoided by a well designed TFF system with an optimized protocol. TFF Process Development The first step in the ... http://www.desmech.com/?p=34 Expanded Bed Adsorption (EBA) allows the protein removal directly from the fermentation broth without going for prior removal of particulates like cell debris and other unwanted products. It reduces the time and cost for further separation procedures in the down stream processing. We can eliminate the cell removal and ... http://www.desmech.com/?p=33 Biofuel has become the buzz of the energy industry. Until now, we have posted on Biopharmaceutical and Material handling. A few posts related to Biofuel shall follow. The relationship between BioPharma and Biofuel shall also be discussed. Biofuel is the type of fuel that is derived from the living ... http://www.desmech.com/?p=32 Cell disruption is needed for the extraction of intracellular products. The method used may vary depending on the type of cell and its cell wall composition. Irrespective of the method used, the main aim is that the disruption must be effective and the method should not be too harsh so ... http://www.desmech.com/?p=28 Disruption is to disorder something, or to break it apart. This interpretation may be extended easily to the disruption of microbial cells. The desired product is located intracellular and is isolated from the suspending medium by an impermeable cell wall and membrane. Cell disruption is the breaking apart of this ... http://www.desmech.com/?p=26 Filtration is the process of separating the components of a solution based on their size and charge differences using a filter. It is a pressure driven process. Filtration is an important process in biopharmaceutical industry which is used right from the separation of cell mass to the final concentration of ... http://www.desmech.com/?p=29 In continuation to an earlier description about what a CIP system is, the CIP procedure follows The CIP Procedure: CIP (Cleaning In Place) is usually accomplished through the action of chemicals by spraying or pressure re-circulating the flushing, washing, and rinsing solutions under the controlled conditions of time, temperature and ... http://www.desmech.com/?p=21 As discussed in the previous topics, the CIP or Cleaning in Place is today, a mandatory requirement in any process industry especially in pharmaceutical and biopharmaceutical. The case in Pharma and BioPharma is very severe because of contamination issues and regulatory conformance. CIP must be validated to ensure proper cleaning. ... http://www.desmech.com/?p=24 Material Handling is a very wide topic but is the nerve of any industry. Material handling includes activities carried out to move any component, raw material, finished goods, equipment or as such anything. Commonly one could see the coolie carrying head loads. This is the most common example of material ... http://www.desmech.com/?p=23 FLUSH BOTTOM VALVE / TANK OUTLET VALVE Valves used to drain off the contents of a tank or vessel or equipment is termed as (Tank) Bottom Valve. What does a Tank Bottom Valve do? • Provide drainage. • Provide Dead space free shutoff. Which is a Tank Bottom Valve? • Any valve serving the purpose. • Can be manual or ... http://www.desmech.com/?p=16