Cell disruption is needed for the extraction of intracellular products. The method used may vary depending on the type of cell and its cell wall composition. Irrespective of the method used, the main aim is that the disruption must be effective and the method should not be too harsh so that the product recovered remains in its active form.
There are different Read the rest of this post »
Disruption is to disorder something, or to break it apart. This interpretation may be extended easily to the disruption of microbial cells. The desired product is located intracellular and is isolated from the suspending medium by an impermeable cell wall and membrane. Cell disruption Read the rest of this post »
Filtration is the process of separating the components of a solution based on their size and charge differences using a filter. It is a pressure driven process. Filtration is an important process in biopharmaceutical industry which is used right from the separation of cell mass to the final concentration of the product.
Types of Filtration
Based on the fluid flow, filtration can be Read the rest of this post »
Tangential Flow Filtration (TFF) is used extensively in biopharmaceutical industries for clarification of microbial cells/animal cells, inclusion bodies clarification, protein concentration and diafiltration, buffer exchange, viral clearance, etc. Multiple Centrifugation steps can be avoided by a well designed TFF system with an optimized protocol.
TFF Process Development
The first step in the development of a TFF Read the rest of this post »